我们矢志于为追求精致英译的翻译员助上一臂之力。集千万句库为你所用，借万千智慧任尔调配，通译句典——为攀登翻译高峰另辟蹊径！
双语句库分类列表：
化学:化学习题:
Once again, you centrifuge the sample, but this time you save the pellet because it contains the protein of interest. What is the rationale for the two-step addition of the salt?
同样，你离心样品，但这次你保留沉淀，因为它含感兴趣的蛋白质。两步加硫酸铵的基本原理是什么?
属类：化学及生命科学-化学-化学习题
You solubilize the ammonium sulfate pellet containing the mitochondrial proteins and dialyze it overnight against large volumes of buffered (pH 7.2)solution.
你把含线粒体蛋白的硫酸铵沉淀溶解，用大体积的缓冲了的(pH7.2)溶液透析过夜。
属类：化学及生命科学-化学-化学习题
Why isn’t ammonium sulfate included in the dialysis buffer? Why do you use the buffer solution instead of water?
为什么此步中透析缓冲液中不加硫酸铵?为什么使用缓冲液而不用水?
属类：化学及生命科学-化学-化学习题
You run the dialyzed solution over a size-exclusion chromatographic column. Following the protocol, you collect, the first protein fraction that exits the column, and discard the rest of the fractions that elute from the column later.
你将透析了的溶液进行分子排阻层析。根据方法，你收集柱子上出来的第一个蛋白质组分，弃去后来流出的其它蛋白质组分。
属类：化学及生命科学-化学-化学习题
You detect the protein by measuring UV absorption (at 280 nm)in the fractions. What does the instruction to collect the first fraction tell you about the protein?
你检测各组分蛋白质是通过紫外吸收(280nm)。收集第一个蛋白质组分这一指令告诉你关于这个蛋白质的什么情况?
属类：化学及生命科学-化学-化学习题
Why is UV absorption at 280 nm a good way to monitor for the presence of protein in the eluted fractions?
为什么280nm紫外吸收是检测洗脱组分中蛋白质的存在的好方法?
属类：化学及生命科学-化学-化学习题
You place the fraction collected in on a cation-exchange chromatographic column.
你把(e)步中收集到的组分进行阳离子交换。
属类：化学及生命科学-化学-化学习题
After discarding the initial solution that exits the column(the flowthrough), you add a washing solution of higher pH to the column and collect the protein fraction that immediately elutes.
当除去开始流出柱子的溶液后，你在柱中加较高pH的洗脱液，然后立即收集流出液。
属类：化学及生命科学-化学-化学习题
You run a small sample of your fraction, now very reduced in volume and quite clear (though tinged pink)on an isoelectric focusing gel. When stained, the gel shows three sharp bands.
你在等电聚焦胶上,跑少量的非常透明的所得样品。染色后，胶上表现出三条清晰的带。
属类：化学及生命科学-化学-化学习题
According to the protocol, the protein of interest is the one with the pI of 5.6 , but you decide to do one more assay of the protein’s purity.
根据方法，感兴趣的蛋白质的pI为5.6,但你决定再测一下此蛋白质的纯度。
属类：化学及生命科学-化学-化学习题
You cut out the pI 5.6 band and subject it to SDS-polyacrylamide gel electrophoresis. The protein resolves as a single band.
你把pI为5.6的带切下，进行SDS-PAGE.此一蛋白表现出一单带。
属类：化学及生命科学-化学-化学习题
Why were you unconvinced of the purity of a "single" protein band, on your IEF gel? What did the results of the SDS gel tell you? Why is it important to do the SDS gel electrophoresis after the isoelectric focusing?
为什么你不能确认你的IEF胶上的“单带”是纯?SDS胶的结果告诉你什么?为什么在IEF后还要跑SDS-PAGE?
属类：化学及生命科学-化学-化学习题
InX-ray studies of crystalline peptides, Linus Pauling and Robert Corey found that the C-N bond in the peptide link is intermediate in length (1.32 A)between a typical C-N single bond (1.49 ?)and a C=N double bond (1.27 ?).
在晶体肽X-射线衍射研究中，Linus Pauling和Rbert Corey发现，在肽键中C-N键的键长介于典型的C-N单键(1.49?)和C=N双键(1.27?)之间。
属类：化学及生命科学-化学-化学习题
They also found that the peptide bond planar (all four atoms attached to the C-N group are located in the same plane)and that the two α-C atoms attached to the C-N are always trans to other (on opposite sides of the peptide bond)
他们还发现，肽键是平面的(与C-N相连的所有四个原子在同一个平面内)，与C-N相连的两个α-C原子总是互为反式的(在肽键的相对面)
属类：化学及生命科学-化学-化学习题
What does the length of the C-N bond in the peptide linkage indicate about its strength and its bond order (i.e., whether it is single, double, or triple)?
肽键中C-N键的键长说明其强度和键级(单、双或三键)的什么情况?
属类：化学及生命科学-化学-化学习题
What do the observations of Pauling and Corey tell us about, the ease of rotation about the C-N peptide bond?
Linus Pauling和Rbert Corey的观察告诉我们C-N肽键转动的什么情况?
属类：化学及生命科学-化学-化学习题
William Astbury discovered that the X-ray pattern of wool shows a repeating structural unit spaced about 5.2 ? along the direction of the wool fiber.
William Astbury发现，羊毛的X-射线衍射谱表明在羊毛的纤维方向有间距大约5.2?的重复结构单位。
属类：化学及生命科学-化学-化学习题
When he steamed and stretched the wool, the x-ray pattern showed a new repeating structural unit at a spacing of 7.0 ?.
当他蒸煮并拉长羊毛，X-射线衍射谱表现出一个新的大约7.0?间距的重复结构单位。
属类：化学及生命科学-化学-化学习题
Steaming and stretching the wool and then letting it shrink gave an x-ray pattern consistent with the original spacing of about 5.2 ?.
蒸煮并拉长羊毛，然后让其收缩后产生回到间距5.2?。
属类：化学及生命科学-化学-化学习题
Although these observations provided important clues to the molecular structure of wool, Astbury was unable to interpret them at the time.
虽然这些观察提供了羊毛分子结构的重要线索，Astbury那时仍不能解释。
属类：化学及生命科学-化学-化学习题
| 1 | 同样，你离心样品，但这次你保留沉淀，因为它含感兴趣的蛋白质。两步加硫酸铵的基本原理是什么? | Once again, you centrifuge the sample, but this time you save the pellet because it contains the protein of interest. What is the rationale for the two-step addition of the salt? |
|
| 2 | 你把含线粒体蛋白的硫酸铵沉淀溶解，用大体积的缓冲了的(pH7.2)溶液透析过夜。 | You solubilize the ammonium sulfate pellet containing the mitochondrial proteins and dialyze it overnight against large volumes of buffered (pH 7.2)solution. |
|
| 3 | 为什么此步中透析缓冲液中不加硫酸铵?为什么使用缓冲液而不用水? | Why isn’t ammonium sulfate included in the dialysis buffer? Why do you use the buffer solution instead of water? |
|
| 4 | 你将透析了的溶液进行分子排阻层析。根据方法，你收集柱子上出来的第一个蛋白质组分，弃去后来流出的其它蛋白质组分。 | You run the dialyzed solution over a size-exclusion chromatographic column. Following the protocol, you collect, the first protein fraction that exits the column, and discard the rest of the fractions that elute from the column later. |
|
| 5 | 你检测各组分蛋白质是通过紫外吸收(280nm)。收集第一个蛋白质组分这一指令告诉你关于这个蛋白质的什么情况? | You detect the protein by measuring UV absorption (at 280 nm)in the fractions. What does the instruction to collect the first fraction tell you about the protein? |
|
| 6 | 为什么280nm紫外吸收是检测洗脱组分中蛋白质的存在的好方法? | Why is UV absorption at 280 nm a good way to monitor for the presence of protein in the eluted fractions? |
|
| 7 | 你把(e)步中收集到的组分进行阳离子交换。 | You place the fraction collected in on a cation-exchange chromatographic column. |
|
| 8 | 当除去开始流出柱子的溶液后，你在柱中加较高pH的洗脱液，然后立即收集流出液。 | After discarding the initial solution that exits the column(the flowthrough), you add a washing solution of higher pH to the column and collect the protein fraction that immediately elutes. |
|
| 9 | 你在等电聚焦胶上,跑少量的非常透明的所得样品。染色后，胶上表现出三条清晰的带。 | You run a small sample of your fraction, now very reduced in volume and quite clear (though tinged pink)on an isoelectric focusing gel. When stained, the gel shows three sharp bands. |
|
| 10 | 根据方法，感兴趣的蛋白质的pI为5.6,但你决定再测一下此蛋白质的纯度。 | According to the protocol, the protein of interest is the one with the pI of 5.6 , but you decide to do one more assay of the protein’s purity. |
|
| 11 | 你把pI为5.6的带切下，进行SDS-PAGE.此一蛋白表现出一单带。 | You cut out the pI 5.6 band and subject it to SDS-polyacrylamide gel electrophoresis. The protein resolves as a single band. |
|
| 12 | 为什么你不能确认你的IEF胶上的“单带”是纯?SDS胶的结果告诉你什么?为什么在IEF后还要跑SDS-PAGE? | Why were you unconvinced of the purity of a "single" protein band, on your IEF gel? What did the results of the SDS gel tell you? Why is it important to do the SDS gel electrophoresis after the isoelectric focusing? |
|
| 13 | 在晶体肽X-射线衍射研究中，Linus Pauling和Rbert Corey发现，在肽键中C-N键的键长介于典型的C-N单键(1.49?)和C=N双键(1.27?)之间。 | InX-ray studies of crystalline peptides, Linus Pauling and Robert Corey found that the C-N bond in the peptide link is intermediate in length (1.32 A)between a typical C-N single bond (1.49 ?)and a C=N double bond (1.27 ?). |
|
| 14 | 他们还发现，肽键是平面的(与C-N相连的所有四个原子在同一个平面内)，与C-N相连的两个α-C原子总是互为反式的(在肽键的相对面) | They also found that the peptide bond planar (all four atoms attached to the C-N group are located in the same plane)and that the two α-C atoms attached to the C-N are always trans to other (on opposite sides of the peptide bond) |
|
| 15 | 肽键中C-N键的键长说明其强度和键级(单、双或三键)的什么情况? | What does the length of the C-N bond in the peptide linkage indicate about its strength and its bond order (i.e., whether it is single, double, or triple)? |
|
| 16 | Linus Pauling和Rbert Corey的观察告诉我们C-N肽键转动的什么情况? | What do the observations of Pauling and Corey tell us about, the ease of rotation about the C-N peptide bond? |
|
| 17 | William Astbury发现，羊毛的X-射线衍射谱表明在羊毛的纤维方向有间距大约5.2?的重复结构单位。 | William Astbury discovered that the X-ray pattern of wool shows a repeating structural unit spaced about 5.2 ? along the direction of the wool fiber. |
|
| 18 | 当他蒸煮并拉长羊毛，X-射线衍射谱表现出一个新的大约7.0?间距的重复结构单位。 | When he steamed and stretched the wool, the x-ray pattern showed a new repeating structural unit at a spacing of 7.0 ?. |
|
| 19 | 蒸煮并拉长羊毛，然后让其收缩后产生回到间距5.2?。 | Steaming and stretching the wool and then letting it shrink gave an x-ray pattern consistent with the original spacing of about 5.2 ?. |
|
| 20 | 虽然这些观察提供了羊毛分子结构的重要线索，Astbury那时仍不能解释。 | Although these observations provided important clues to the molecular structure of wool, Astbury was unable to interpret them at the time. |
|
查询记录
